[G-3H] Gougerotin Binding to Ribosomes. Heterogeneity of Eukaryotic Ribosomes

Magnesium binding to yeast ribosomes.

Recent mechanistic insights into eukaryotic ribosomes.

Ribosomes are supramolecular ribonucleoprotein particles that synthesize proteins in all cells. Protein synthesis proceeds through four major phases: initiation, elongation, termination, and ribosome recycling. In each phase, a number of phase-specific translation factors cooperate with the ribosome. Whereas elongation in prokaryotes and eukaryotes involve similar factors and proceed by similar...

Binding of erythromycin to Escherichia coli ribosomes.

This paper concerns a precise study on the nature of the binding of erythromycin to Escherichia coli (Q13) ribosomes. (1) An equimolar amount of erythromycin bound to 50S ribosomal subunits at extremely low concentrations and this binding was not affected by the addition of 30S ribosomal subunits. (2) The binding of erythromycin to ribosomes was not significantly influenced by decreasing the Mg...

[3H] dihydrostreptomycin accumulation and binding to ribosomes in Rhizobium mutants with different levels of streptomycin resistance.

Rhizobium trifolii B1, a symbiotic nitrogen fixer, is sensitive to streptomycin (10 microgram/ml) and spontaneously produces spheroplast-like forms during cultivation. Streptomycin-resistant mutants selected with high doses of antibiotic (1,000 microgram/ml) showed pleiotropic changes, including loss of spheroplast formation and infectivity to plants, whereas mutants selected with low doses of ...

Ribosomal proteins S9 and L6 participate in the binding of [3H]dibekacin to E. coli ribosomes.

The degrees of binding of [3H]dibekacin to LiCl-treated cores of E. coli ribosomes were reduced by increasing LiCl concentrations. The 1.15 M LiCl core lost 70 approximately 80% of the original binding capacity. The antibiotic attachment to the 1.15 M LiCl core was restored by reconstitution with the split proteins (SP), which were obtained by the treatment of 70S ribosomes with LiCl at concent...